LL-37 Dosing Calculator
LL-37: Human cathelicidin antimicrobial peptide guide.
Pre-filled with the most-reported community reconstitution protocol for LL-37. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Also available in these vial strengths
Same LL-37 protocol, recalculated for each vial size — reconstitution volumes, concentrations, and syringe-draw units update automatically.
Quick answer · Community-reported standard
A 2 mg vial of LL-37 reconstituted with 1 mL of bacteriostatic water yields a concentration of 1.00 mg/mL. At that concentration, a 100 mcg dose of LL-37 equals 10 units on a U-100 insulin syringe (0.100 mL). LL-37 is typically administered twice daily via subcutaneous.
LL-37 2 mg reconstitution and dosage: 1 mL vs 2 mL bac water
For a 2 mg vial of LL-37, bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 2 mg vial.
| BAC water added | Concentration | Volume per 100 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 2.00 mg/mL | 0.050 mL | 5 units |
| 2 mL | 1.00 mg/mL | 0.100 mL | 10 units |
LL-37 dosage chart: per-injection to weekly total
Common LL-37 protocol doses centered on the 100 mcg baseline (research range 100 mcg–200 mcg), with weekly totals at twice daily frequency.
| Dose level | Per injection | Frequency | Weekly total |
|---|---|---|---|
| Lower | 0.05 mg | Twice daily | 0.7 mg |
| Baseline | 0.1 mg | Twice daily | 1.4 mg |
| Higher | 0.15 mg | Twice daily | 2.1 mg |
| Upper range | 0.2 mg | Twice daily | 2.8 mg |
Weekly totals assume consistent twice daily dosing. Adjust the calculator below for your specific vial size and dose.
Calculate your LL-37 dose
Alternative LL-37 protocols
Beyond the default above, LL-37 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Acute Infection Protocol
Community- Dose
- 200-400 mcg
- Frequency
- Once daily
- Duration
- 2-4 weeks
- Route
- Subcutaneous
Higher dose used during active infections; some users report flu-like symptoms at higher doses
Gut Repair Protocol
Community- Dose
- 100 mcg
- Frequency
- Once daily
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Lower dose used for gut-related issues; often stacked with BPC-157 for gut healing
Slow Titration Protocol
Community- Dose
- 50-400 mcg (gradual increase)
- Frequency
- Once daily
- Duration
- 8-16 weeks
- Route
- Subcutaneous
Start at 50 mcg, increase by 50 mcg weekly as tolerated; targeting 200-400 mcg daily by weeks 4-8
Antimicrobial and immune support (subcutaneous)
Clinical- Dose
- 100-200 mcg
- Frequency
- Once daily, 5 days per week
- Duration
- 2-4 weeks on, 2 weeks off
Based on anecdotal protocols; no established human SC dosing from clinical trials
Wound healing (topical)
Clinical- Dose
- 0.5 mg/mL topical formulation
- Frequency
- Twice weekly application to wound site
- Duration
- 4-13 weeks depending on indication
Based on Phase IIb venous leg ulcer trial (Mahlapuu 2021) and DFU trial (Miranda 2023)
LL-37 reconstitution & storage
Reconstitute lyophilized LL-37 with bacteriostatic water. Add water slowly along the vial wall to avoid foaming. Allow to dissolve without shaking. Store reconstituted solution refrigerated.
Store lyophilized peptide at -20C for long-term storage. Reconstituted solution should be refrigerated at 2-8C and used within 2-3 weeks. Protect from light and repeated freeze-thaw cycles.
LL-37 dosing FAQ
How much bacteriostatic water should I add to a 2 mg vial of LL-37?
The community standard is 1 mL of bacteriostatic water for a 2 mg LL-37 vial. That gives a concentration of 1.00 mg/mL, so a 100 mcg dose is 10 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 2.00 mg/mL and halves the draw to 5 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 100 mcg dose of LL-37?
On a standard U-100 insulin syringe, a 100 mcg dose of LL-37 is 10 units when the 2 mg vial is reconstituted with 1 mL of bacteriostatic water (1.00 mg/mL). If you use 1 mL of bac water instead, draw 5 units (2.00 mg/mL).
What is the standard LL-37 dose?
Community-reported protocols for LL-37 typically use 100 mcg–200 mcg twice daily via Subcutaneous. 100 mcg is a common starting dose. Most commonly reported protocol; gradual titration from 50 mcg recommended
How long does a vial of LL-37 last at 100 mcg twice daily?
A 2 mg vial of LL-37 contains roughly 20 doses at 100 mcg per injection. At twice daily dosing, that is approximately 10 days (about 1 weeks) of supply per vial. Reconstituted LL-37 should generally be used within 28 days when refrigerated.
Where do you inject LL-37?
LL-37 is typically injected subcutaneous. Common sites reported in the literature and by users include: Subcutaneous injection in abdominal area, Subcutaneous injection in upper thigh. Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for LL-37?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like LL-37 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on LL-37
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.