LL-37 Dosing Calculator
LL-37: Human cathelicidin antimicrobial peptide guide.
Pre-filled with the most-reported community reconstitution protocol for LL-37. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 5 mg vial of LL-37 reconstituted with 1 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 100 mcg dose of LL-37 equals 4 units on a U-100 insulin syringe (0.040 mL). LL-37 is typically administered twice daily via subcutaneous.
LL-37 reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 100 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.020 mL | 2 units |
| 2 mL | 2.50 mg/mL | 0.040 mL | 4 units |
Calculate your LL-37 dose
Alternative LL-37 protocols
Beyond the default above, LL-37 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Acute Infection Protocol
Community- Dose
- 200-400 mcg
- Frequency
- Once daily
- Duration
- 2-4 weeks
- Route
- Subcutaneous
Higher dose used during active infections; some users report flu-like symptoms at higher doses
Gut Repair Protocol
Community- Dose
- 100 mcg
- Frequency
- Once daily
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Lower dose used for gut-related issues; often stacked with BPC-157 for gut healing
Slow Titration Protocol
Community- Dose
- 50-400 mcg (gradual increase)
- Frequency
- Once daily
- Duration
- 8-16 weeks
- Route
- Subcutaneous
Start at 50 mcg, increase by 50 mcg weekly as tolerated; targeting 200-400 mcg daily by weeks 4-8
Antimicrobial and immune support (subcutaneous)
Clinical- Dose
- 100-200 mcg
- Frequency
- Once daily, 5 days per week
- Duration
- 2-4 weeks on, 2 weeks off
Based on anecdotal protocols; no established human SC dosing from clinical trials
Wound healing (topical)
Clinical- Dose
- 0.5 mg/mL topical formulation
- Frequency
- Twice weekly application to wound site
- Duration
- 4-13 weeks depending on indication
Based on Phase IIb venous leg ulcer trial (Mahlapuu 2021) and DFU trial (Miranda 2023)
LL-37 reconstitution & storage
Reconstitute lyophilized LL-37 with bacteriostatic water. Add water slowly along the vial wall to avoid foaming. Allow to dissolve without shaking. Store reconstituted solution refrigerated.
Store lyophilized peptide at -20C for long-term storage. Reconstituted solution should be refrigerated at 2-8C and used within 2-3 weeks. Protect from light and repeated freeze-thaw cycles.
LL-37 dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of LL-37?
The community standard is 1 mL of bacteriostatic water for a 5 mg LL-37 vial. That gives a concentration of 2.50 mg/mL, so a 100 mcg dose is 4 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 2 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 100 mcg dose of LL-37?
On a standard U-100 insulin syringe, a 100 mcg dose of LL-37 is 4 units when the 5 mg vial is reconstituted with 1 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 2 units (5.00 mg/mL).
What is the standard LL-37 dose?
Community-reported protocols for LL-37 typically use 100 mcg–200 mcg twice daily via Subcutaneous. 100 mcg is a common starting dose. Most commonly reported protocol; gradual titration from 50 mcg recommended
How long does a vial of LL-37 last at 100 mcg twice daily?
A 5 mg vial of LL-37 contains roughly 50 doses at 100 mcg per injection. At twice daily dosing, that is approximately 25 days (about 4 weeks) of supply per vial. Reconstituted LL-37 should generally be used within 28 days when refrigerated.
Where do you inject LL-37?
LL-37 is typically injected subcutaneous. Common sites reported in the literature and by users include: Subcutaneous injection in abdominal area, Subcutaneous injection in upper thigh. Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for LL-37?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like LL-37 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on LL-37
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.