TB500 Dosing Calculator
TB-500: Thymosin beta-4 guide for tissue repair and healing.
Pre-filled with the most-reported community reconstitution protocol for TB500. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 10 mg vial of TB500 reconstituted with 2 mL of bacteriostatic water yields a concentration of 5.00 mg/mL. At that concentration, a 4 mg dose of TB500 equals 80 units on a U-100 insulin syringe (0.800 mL). TB500 is typically administered once daily via subcutaneous.
TB500 reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 10 mg vial.
| BAC water added | Concentration | Volume per 4 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 10.00 mg/mL | 0.400 mL | 40 units |
| 2 mL | 5.00 mg/mL | 0.800 mL | 80 units |
Calculate your TB500 dose
Alternative TB500 protocols
Beyond the default above, TB500 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Standard Recovery Protocol
Community- Dose
- 2-5 mg
- Frequency
- Twice weekly
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Used for general injury recovery; fixed twice-weekly dosing without a distinct loading phase
Low-Dose Healing Protocol
Community- Dose
- 2 mg
- Frequency
- Twice weekly
- Duration
- 4-6 weeks
- Route
- Subcutaneous
Conservative approach for minor injuries and joint discomfort; some users report good results at lower doses
Aggressive Injury Protocol
Community- Dose
- 5-10 mg
- Frequency
- 2-3 times weekly
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Used for severe injuries or post-surgical recovery; higher doses than most community members use
Phase I clinical trial (IV, safety assessment)
Clinical- Dose
- 42-1260 mg IV
- Frequency
- Once daily
- Duration
- 14 days (after single-dose phase)
Phase I study in healthy volunteers. Four ascending dose cohorts (42, 140, 420, 1260 mg) administered intravenously. Well tolerated at all dose levels with no dose-limiting toxicities.
Dermal wound healing (topical, clinical trial)
Clinical- Dose
- 0.03% topical gel
- Frequency
- Applied to wound site per protocol
- Duration
- Until wound closure or study endpoint
Phase II trial for venous stasis ulcers (NCT00832091). Topical application directly to wound bed. Accelerated healing by approximately one month in responders.
TB500 reconstitution & storage
Reconstitute lyophilized TB500 with bacteriostatic water (0.9% benzyl alcohol preserved). For a 5 mg vial, add 1-2 mL of bacteriostatic water for a concentration of 2.5-5 mg/mL. Direct the stream of water against the glass wall of the vial, not directly onto the lyophilized cake. Gently swirl (do not shake) until fully dissolved. The solution should be clear and colorless. If particulates or discoloration are observed, do not use.
Store lyophilized (unreconstituted) vials at -20C for long-term storage or 2-8C (refrigerator) for short-term. Reconstituted solution should be refrigerated at 2-8C and used within 14-28 days. Do not freeze reconstituted solution. Protect from light. Avoid repeated freeze-thaw cycles. Discard if solution becomes cloudy, discolored, or contains visible particulates.
TB500 dosing FAQ
How much bacteriostatic water should I add to a 10 mg vial of TB500?
The community standard is 2 mL of bacteriostatic water for a 10 mg TB500 vial. That gives a concentration of 5.00 mg/mL, so a 4 mg dose is 80 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 10.00 mg/mL and halves the draw to 40 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 4 mg dose of TB500?
On a standard U-100 insulin syringe, a 4 mg dose of TB500 is 80 units when the 10 mg vial is reconstituted with 2 mL of bacteriostatic water (5.00 mg/mL). If you use 1 mL of bac water instead, draw 40 units (10.00 mg/mL).
What is the standard TB500 dose?
Community-reported protocols for TB500 typically use 4 mgâ8 mg once daily via Subcutaneous. 4 mg is a common starting dose. Most commonly reported protocol; loading phase of 6-8 mg/week split into 2-3 injections, then tapering to 2-4 mg every 1-2 weeks
How long does a vial of TB500 last at 4 mg once daily?
A 10 mg vial of TB500 contains roughly 2 doses at 4 mg per injection. At once daily dosing, that is approximately 2 days (about 0 weeks) of supply per vial. Reconstituted TB500 should generally be used within 28 days when refrigerated.
Where do you inject TB500?
TB500 is typically injected subcutaneous. Common sites reported in the literature and by users include: Abdomen (subcutaneous, most common research site), Thigh (subcutaneous), Upper arm/deltoid region (subcutaneous), Near site of injury (subcutaneous, localized protocols). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for TB500?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like TB500 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on TB500
TB500 overview
Mechanism of action, primary uses, and research summary
TB500 dosing protocols
Full clinical and research-based dosing reference
TB500 community protocols
Real-world usage patterns from peptide communities
TB500 side effects
Reported adverse effects and safety considerations
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Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.