PE-22-28 Dosing Calculator
PE-22-28: synthetic spadin analog TREK-1 potassium channel blocker.
Pre-filled with the most-reported community reconstitution protocol for PE-22-28. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Also available in these vial strengths
Same PE-22-28 protocol, recalculated for each vial size — reconstitution volumes, concentrations, and syringe-draw units update automatically.
Quick answer · Community-reported standard
A 2 mg vial of PE-22-28 reconstituted with 1 mL of bacteriostatic water yields a concentration of 1.00 mg/mL. At that concentration, a 100 mcg dose of PE-22-28 equals 10 units on a U-100 insulin syringe (0.100 mL). PE-22-28 is typically administered once daily via subcutaneous.
PE-22-28 2 mg reconstitution and dosage: 1 mL vs 2 mL bac water
For a 2 mg vial of PE-22-28, bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 2 mg vial.
| BAC water added | Concentration | Volume per 100 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 2.00 mg/mL | 0.050 mL | 5 units |
| 2 mL | 1.00 mg/mL | 0.100 mL | 10 units |
PE-22-28 dosage chart: per-injection to weekly total
Common PE-22-28 protocol doses centered on the 100 mcg baseline (research range 100 mcg–200 mcg), with weekly totals at once daily frequency.
| Dose level | Per injection | Frequency | Weekly total |
|---|---|---|---|
| Lower | 0.05 mg | Once daily | 0.35 mg |
| Baseline | 0.1 mg | Once daily | 0.7 mg |
| Higher | 0.15 mg | Once daily | 1.05 mg |
| Upper range | 0.2 mg | Once daily | 1.4 mg |
Weekly totals assume consistent once daily dosing. Adjust the calculator below for your specific vial size and dose.
Calculate your PE-22-28 dose
Alternative PE-22-28 protocols
Beyond the default above, PE-22-28 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Standard Intranasal Protocol
Community- Dose
- 200-400 mcg
- Frequency
- Once daily, morning
- Duration
- 4-8 weeks
- Route
- Intranasal
Most commonly reported protocol; morning dosing preferred for mood and cognitive effects throughout the day
Titration Protocol
Community- Dose
- 100 mcg starting, titrate to 150-200 mcg
- Frequency
- Once daily
- Duration
- 8-16 weeks with gradual titration
- Route
- Subcutaneous
Conservative approach starting low and increasing based on response; recommended by some community guides
Acute Antidepressant Effect (Djillani et al., 2017)
Clinical- Dose
- 3-4 mcg/kg intraperitoneal
- Frequency
- Single dose
- Duration
- Acute
Reduced immobility time in forced swimming test. Duration of action approximately 23 hours after single injection.
Sub-Chronic Neurogenesis Protocol (Djillani et al., 2017)
Clinical- Dose
- 3-4 mcg/kg/day intraperitoneal
- Frequency
- Daily
- Duration
- 4 days
Induced hippocampal neurogenesis and enhanced synaptogenesis (increased PSD-95 expression) after only 4 days of treatment in mice.
PE-22-28 reconstitution & storage
Reconstitute lyophilized PE-22-28 in sterile water or bacteriostatic water. Typical research concentrations are 0.1-1 mg/mL. Store reconstituted solution refrigerated or frozen.
Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be refrigerated at 2-8 degrees C and used within 4 weeks, or aliquoted and frozen at -20 degrees C.
PE-22-28 dosing FAQ
How much bacteriostatic water should I add to a 2 mg vial of PE-22-28?
The community standard is 1 mL of bacteriostatic water for a 2 mg PE-22-28 vial. That gives a concentration of 1.00 mg/mL, so a 100 mcg dose is 10 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 2.00 mg/mL and halves the draw to 5 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 100 mcg dose of PE-22-28?
On a standard U-100 insulin syringe, a 100 mcg dose of PE-22-28 is 10 units when the 2 mg vial is reconstituted with 1 mL of bacteriostatic water (1.00 mg/mL). If you use 1 mL of bac water instead, draw 5 units (2.00 mg/mL).
What is the standard PE-22-28 dose?
Community-reported protocols for PE-22-28 typically use 100 mcg–200 mcg once daily via Subcutaneous. 100 mcg is a common starting dose. SubQ injection as alternative to intranasal; some users report stronger effects but higher risk of lethargy at higher doses
How long does a vial of PE-22-28 last at 100 mcg once daily?
A 2 mg vial of PE-22-28 contains roughly 20 doses at 100 mcg per injection. At once daily dosing, that is approximately 20 days (about 3 weeks) of supply per vial. Reconstituted PE-22-28 should generally be used within 28 days when refrigerated.
Where do you inject PE-22-28?
PE-22-28 is typically injected subcutaneous. Common sites reported in the literature and by users include: Intraperitoneal (animal research), Subcutaneous (animal research). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for PE-22-28?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like PE-22-28 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on PE-22-28
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.