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Pinealon Dosing Calculator

Pinealon (EDR): Bioregulatory neuroprotective tripeptide.

Pre-filled with the most-reported community reconstitution protocol for Pinealon. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.

Quick answer · Community-reported standard

A 5 mg vial of Pinealon reconstituted with 1 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 100 mcg dose of Pinealon equals 4 units on a U-100 insulin syringe (0.040 mL). Pinealon is typically administered once daily via subcutaneous.

Pinealon reconstitution: 1 mL vs 2 mL bac water

Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.

BAC water addedConcentrationVolume per 100 mcg doseDraw on U-100 syringe (units)
1 mL5.00 mg/mL0.020 mL2 units
2 mL2.50 mg/mL0.040 mL4 units
Based on a 5 mg vial of Pinealon reconstituted with bacteriostatic water. Insulin-syringe units assume a standard U-100 syringe (100 units = 1 mL).

Calculate your Pinealon dose

Alternative Pinealon protocols

Beyond the default above, Pinealon is also reported in the following protocols. Use these as starting points and recalculate with the tool above.

Oral Capsule Protocol

Community
Dose
1-2 capsules (typically 10 mg each)
Frequency
1-2 times daily with meals
Duration
10-30 days, repeated every 4-6 months
Route
Oral

Most accessible format; taken with food, morning or early afternoon preferred for cognitive effects

Intranasal Spray Protocol

Community
Dose
2-4 mg
Frequency
Once daily
Duration
10-20 days
Route
Intranasal

Nasal spray for direct CNS delivery; considered by some users to be the most effective route for cognitive effects

Neuroprotection (preclinical reference dose from rat ischemia model)

Clinical
Dose
10 mcg/kg intraperitoneally (animal dose; human equivalent not established)
Frequency
Once daily
Duration
5 days prior to insult (preventive regimen used in rat study)

Only single-dose level studied in vivo; no human dose-finding trials completed

Oral supplementation (from manufacturer guidelines, not clinically validated)

Clinical
Dose
200-500 mcg subcutaneously or 10-20 mg orally per capsule
Frequency
Once daily (SC) or as directed (oral), 5 days per week
Duration
30 days per course, repeated every 4-6 months

Based on manufacturer recommendations; no controlled human trials support these doses

Pinealon reconstitution & storage

Not typically required for oral capsule form; if using lyophilized powder for subcutaneous injection, reconstitute with bacteriostatic water per standard peptide practice

Store lyophilized powder at -20C or below; reconstituted solution at 2-8C; no Pinealon-specific stability data available

Pinealon dosing FAQ

How much bacteriostatic water should I add to a 5 mg vial of Pinealon?

The community standard is 1 mL of bacteriostatic water for a 5 mg Pinealon vial. That gives a concentration of 2.50 mg/mL, so a 100 mcg dose is 4 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 2 units. Less water = smaller injection volume but harder to measure precisely.

How many syringe units is a 100 mcg dose of Pinealon?

On a standard U-100 insulin syringe, a 100 mcg dose of Pinealon is 4 units when the 5 mg vial is reconstituted with 1 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 2 units (5.00 mg/mL).

What is the standard Pinealon dose?

Community-reported protocols for Pinealon typically use 100 mcg–300 mcg once daily via Subcutaneous. 100 mcg is a common starting dose. Injection route used by those seeking more precise dosing; administered in the morning

How long does a vial of Pinealon last at 100 mcg once daily?

A 5 mg vial of Pinealon contains roughly 50 doses at 100 mcg per injection. At once daily dosing, that is approximately 50 days (about 7 weeks) of supply per vial. Reconstituted Pinealon should generally be used within 28 days when refrigerated.

Where do you inject Pinealon?

Pinealon is typically injected subcutaneous. Common sites reported in the literature and by users include: Abdomen, Outer thigh. Rotate sites with each injection to reduce localized irritation.

Can I use sterile water instead of bacteriostatic water for Pinealon?

Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Pinealon because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.

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Medical Disclaimer

This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.