PE-22-28: Dosing Protocols
Dosing guidelines, reconstitution, and administration information
๐TL;DR
- โข2 dosing protocols documented
- โขReconstitution instructions included
- โขStorage: Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be refrigerated at 2-8 degrees C and used within 4 weeks, or aliquoted and frozen at -20 degrees C.
Protocol Quick-Reference
Antidepressant and neurogenesis research
Dosing
Amount
3-4 mcg/kg
Frequency
Daily
Duration
4 days (sub-chronic neurogenesis protocol)
Administration
Route
SCSchedule
Once daily
Timing
Preclinical data only; administered intraperitoneally in mice; subcutaneous route used in animal research
Cycle
Duration
4 days
Repeatable
Yes
โ๏ธ Suggested Bloodwork (2 tests)
CBC with differential
When: Baseline
Why: Baseline blood cell counts
CMP (Comprehensive Metabolic Panel)
When: Baseline
Why: Liver and kidney function baseline
๐ก Key Considerations
- โPreclinical compound only: no human clinical trials have been conducted
- โAll dosing data derived from mouse studies; human dosing has not been established
- โTREK-1 channels have roles beyond mood regulation (pain sensation, neuroprotection): chronic blockade effects are unknown
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| Purpose | Dose | Frequency | Duration | Notes |
|---|---|---|---|---|
| Acute Antidepressant Effect (Djillani et al., 2017) | 3-4 mcg/kg intraperitoneal | Single dose | Acute | Reduced immobility time in forced swimming test. Duration of action approximately 23 hours after single injection. |
| Sub-Chronic Neurogenesis Protocol (Djillani et al., 2017) | 3-4 mcg/kg/day intraperitoneal | Daily | 4 days | Induced hippocampal neurogenesis and enhanced synaptogenesis (increased PSD-95 expression) after only 4 days of treatment in mice. |
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๐Reconstitution Instructions
Reconstitute lyophilized PE-22-28 in sterile water or bacteriostatic water. Typical research concentrations are 0.1-1 mg/mL. Store reconstituted solution refrigerated or frozen.
Recommended Injection Sites
- โIntraperitoneal (animal research)
- โSubcutaneous (animal research)
๐งStorage Requirements
Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be refrigerated at 2-8 degrees C and used within 4 weeks, or aliquoted and frozen at -20 degrees C.
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Before You Begin
Review safety warnings and contraindications before starting any protocol.
Important Disclaimer#
PE-22-28 is not approved for human use by any regulatory agency. There are no established human dosing protocols. All dosing information is derived from preclinical mouse studies and is provided for research reference only.
Research-Based Dosing Information#
Acute Antidepressant Protocol#
Djillani et al. (2017) administered PE-22-28 at 3-4 mcg/kg intraperitoneally as a single dose in mice. This produced significant reduction of immobility time in the forced swimming test, with effects lasting approximately 23 hours (compared to 7 hours for the parent compound spadin).
Sub-Chronic Neurogenesis Protocol#
A 4-day sub-chronic treatment at 3-4 mcg/kg/day IP was sufficient to induce hippocampal neurogenesis and enhance synaptogenesis, as measured by increased PSD-95 expression in mouse cortical neurons. This rapid neurogenic effect compares favorably to traditional antidepressants, which typically require 2-4 weeks to induce neurogenesis.
Dose Considerations#
The effective dose of PE-22-28 is notably low (low microgram per kilogram range), reflecting its extremely high potency at TREK-1 (IC50 0.12 nM). No dose-response studies or maximum tolerated dose studies have been published.
Reconstitution Instructions#
Reconstitute lyophilized PE-22-28 in sterile water or bacteriostatic water. Gently swirl to dissolve. Typical research concentrations range from 0.1 to 1 mg/mL.
Storage Guidelines#
Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be refrigerated and used within 4 weeks, or aliquoted and stored at -20 degrees C.
Dosing Context#
PE-22-28 belongs to the Neuropeptide category of research peptides. Dosing protocols for PE-22-28 are derived from available clinical trial data. These protocols are provided for research reference only and do not constitute medical advice. Actual dosing decisions should be made by qualified healthcare providers based on individual patient factors.
Research Protocols#
The following dosing protocols have been documented in clinical research for PE-22-28:
Acute Antidepressant Effect (Djillani et al., 2017)#
Dose: 3-4 mcg/kg intraperitoneal
Frequency: Single dose
Duration: Acute
Reduced immobility time in forced swimming test. Duration of action approximately 23 hours after single injection.
Sub-Chronic Neurogenesis Protocol (Djillani et al., 2017)#
Dose: 3-4 mcg/kg/day intraperitoneal
Frequency: Daily
Duration: 4 days
Induced hippocampal neurogenesis and enhanced synaptogenesis (increased PSD-95 expression) after only 4 days of treatment in mice.
Reconstitution and Preparation#
Reconstitute lyophilized PE-22-28 in sterile water or bacteriostatic water. Typical research concentrations are 0.1-1 mg/mL. Store reconstituted solution refrigerated or frozen.
Injection Sites#
Recommended injection sites for PE-22-28 include:
- Intraperitoneal (animal research)
- Subcutaneous (animal research)
Site rotation is recommended to minimize local tissue reactions and ensure consistent absorption.
Storage Requirements#
Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be refrigerated at 2-8 degrees C and used within 4 weeks, or aliquoted and frozen at -20 degrees C.
Related Reading#
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Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.