Semax Dosing Calculator
Semax peptide guide: not FDA approved, but used in Russia since 2011.
Pre-filled with the most-reported community reconstitution protocol for Semax. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 5 mg vial of Semax reconstituted with 2 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 300 mcg dose of Semax equals 12 units on a U-100 insulin syringe (0.120 mL). Semax is typically administered once daily via subcutaneous.
Semax reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 300 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.060 mL | 6 units |
| 2 mL | 2.50 mg/mL | 0.120 mL | 12 units |
Calculate your Semax dose
Alternative Semax protocols
Beyond the default above, Semax is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Standard Intranasal Nootropic Protocol
Community- Dose
- 200-600 mcg
- Frequency
- 1-2 times daily
- Duration
- 10-14 days on, 7-14 days off
- Route
- Intranasal
Most commonly reported protocol; 1-2 drops per nostril, morning dosing preferred for cognitive effects
Higher-Dose Cognitive Protocol
Community- Dose
- 600-900 mcg
- Frequency
- 2-3 times daily (split doses)
- Duration
- 10-14 days
- Route
- Intranasal
Used during periods of intense cognitive demand; split dosing throughout the day
NA-Semax Amidate Protocol
Community- Dose
- 200-400 mcg
- Frequency
- 1-2 times daily
- Duration
- 10-14 days on, 14 days off
- Route
- Intranasal
N-Acetyl Semax Amidate variant reportedly more potent and longer-lasting; lower doses used
Cognitive enhancement and nootropic use (Russian approved)
Clinical- Dose
- 200-600 mcg per administration (0.1% solution, 2-6 drops per nostril)
- Frequency
- 2-3 times daily
- Duration
- 10-14 day treatment courses
Based on the approved 0.1% Semax nasal drops (1 mg/mL) in Russia. Each drop delivers approximately 30 mcg. Standard nootropic course is 200-600 mcg per dose, 2-3 times daily for 10-14 days. Courses may be repeated after a 2-4 week break. Used for cognitive fatigue, attention difficulties, and mild cognitive impairment in Russian clinical practice.
Ischemic stroke (acute phase, Russian approved)
Clinical- Dose
- 3000-6000 mcg (3-6 mg) per administration
- Frequency
- 2-4 times daily
- Duration
- 5-14 days
Uses the 1% Semax solution (10 mg/mL). Administered intranasally in the acute period of ischemic stroke. Initiated within hours of symptom onset. Higher doses used for moderate-severity strokes. This is an approved indication in Russia and is administered under medical supervision.
Semax reconstitution & storage
The Russian pharmaceutical product Semax is supplied as a ready-to-use 0.1% or 1% intranasal solution in dropper bottles and does not require reconstitution. For research-grade lyophilized Semax, reconstitute with sterile bacteriostatic water. For a 5 mg vial, add 2.5 mL bacteriostatic water for a concentration of 2 mg/mL. For intranasal research use, dilute to 0.1% (1 mg/mL) or 1% (10 mg/mL) in sterile normal saline with appropriate preservative. Gently swirl to dissolve; do not shake vigorously.
Store lyophilized Semax at -20 degrees Celsius protected from light. The reconstituted Russian pharmaceutical product should be stored at 2-8 degrees Celsius and used within the period specified on the packaging (typically 30 days). Research-grade reconstituted solutions should be refrigerated at 2-8 degrees Celsius and used within 2-4 weeks. Protect from light and oxidizing conditions. The methionine residue is susceptible to oxidation, so minimize exposure to air and UV light.
Semax dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of Semax?
The community standard is 2 mL of bacteriostatic water for a 5 mg Semax vial. That gives a concentration of 2.50 mg/mL, so a 300 mcg dose is 12 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 6 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 300 mcg dose of Semax?
On a standard U-100 insulin syringe, a 300 mcg dose of Semax is 12 units when the 5 mg vial is reconstituted with 2 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 6 units (5.00 mg/mL).
What is the standard Semax dose?
Community-reported protocols for Semax typically use 300 mcg–600 mcg once daily via Subcutaneous. 300 mcg is a common starting dose. Less common route; some users report stronger systemic effects compared to intranasal
How long does a vial of Semax last at 300 mcg once daily?
A 5 mg vial of Semax contains roughly 16 doses at 300 mcg per injection. At once daily dosing, that is approximately 16 days (about 2 weeks) of supply per vial. Reconstituted Semax should generally be used within 28 days when refrigerated.
Where do you inject Semax?
Semax is typically injected subcutaneous. Common sites reported in the literature and by users include: Intranasal (primary route, both nostrils), Subcutaneous (research route). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for Semax?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Semax because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on Semax
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.