Humanin Dosing Calculator

Dose

5-10 mg total per week

Frequency

Divided into 2-3 injections

Duration

2-4 weeks on, 2-4 weeks off

Route

Subcutaneous

Higher dose approach for more pronounced anti-aging effects; divided across multiple weekly injections

Dose

0.5-1 mg

Frequency

Once daily

Duration

4-8 weeks

Route

Subcutaneous

Daily low-dose approach for sustained mitochondrial support; less common due to injection burden

Dose

5 mg/kg/day intraperitoneal

Frequency

Daily

Duration

3 months

Improved spatial memory and reduced amyloid pathology in APPswe/PS1dE9 mice. S14G-humanin analog used, which is 1000-fold more potent than native humanin.

Dose

0.1-10 mcg intracerebroventricular

Frequency

Single or repeated dosing

Duration

Varies by study

Direct CNS delivery used in multiple preclinical models. Native humanin and HNG analog tested at various doses.

How much bacteriostatic water should I add to a 10 mg vial of Humanin?

The community standard is 2 mL of bacteriostatic water for a 10 mg Humanin vial. That gives a concentration of 5.00 mg/mL, so a 1 mg dose is 20 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 10.00 mg/mL and halves the draw to 10 units. Less water = smaller injection volume but harder to measure precisely.

How many syringe units is a 1 mg dose of Humanin?

On a standard U-100 insulin syringe, a 1 mg dose of Humanin is 20 units when the 10 mg vial is reconstituted with 2 mL of bacteriostatic water (5.00 mg/mL). If you use 1 mL of bac water instead, draw 10 units (10.00 mg/mL).

What is the standard Humanin dose?

Community-reported protocols for Humanin typically use 1 mg–5 mg every other day via Subcutaneous. 1 mg is a common starting dose. Uses the potent S14G-humanin (HNG) analog at lower doses than native humanin; most community users prefer HNG

How long does a vial of Humanin last at 1 mg every other day?

A 10 mg vial of Humanin contains roughly 10 doses at 1 mg per injection. At every other day dosing, that is approximately 20 days (about 3 weeks) of supply per vial. Reconstituted Humanin should generally be used within 28 days when refrigerated.

Where do you inject Humanin?

Humanin is typically injected subcutaneous. Common sites reported in the literature and by users include: Intraperitoneal (animal research), Intracerebroventricular (animal research), Subcutaneous (animal research). Rotate sites with each injection to reduce localized irritation.

Can I use sterile water instead of bacteriostatic water for Humanin?

Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Humanin because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.