MOTS-c Dosing Calculator
MOTS-c: Mitochondrial-derived exercise mimetic peptide.
Pre-filled with the most-reported community reconstitution protocol for MOTS-c. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 10 mg vial of MOTS-c reconstituted with 2 mL of bacteriostatic water yields a concentration of 5.00 mg/mL. At that concentration, a 5 mg dose of MOTS-c equals 100 units on a U-100 insulin syringe (1.000 mL). MOTS-c is typically administered every other day via subcutaneous.
MOTS-c reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 10 mg vial.
| BAC water added | Concentration | Volume per 5 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 10.00 mg/mL | 0.500 mL | 50 units |
| 2 mL | 5.00 mg/mL | 1.000 mL | 100 units |
Calculate your MOTS-c dose
Alternative MOTS-c protocols
Beyond the default above, MOTS-c is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Weekly Single-Dose Protocol
Community- Dose
- 10 mg
- Frequency
- Once weekly
- Duration
- Up to 10 weeks
- Route
- Subcutaneous
Single weekly injection timed before endurance exercise for added mitochondrial benefit
Daily Low-Dose Protocol
Community- Dose
- 1 mg
- Frequency
- Once daily
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Daily microdosing approach for more consistent AMPK activation; less common due to injection frequency
Titration Protocol
Community- Dose
- 200 mcg starting, titrate to 1000 mcg
- Frequency
- Once daily
- Duration
- 10 weeks with staged titration
- Route
- Subcutaneous
Conservative staged approach starting at 200 mcg and increasing by 200 mcg every 2 weeks
Preclinical metabolic study (Lee et al. 2015)
Clinical- Dose
- 5 mg/kg body weight
- Frequency
- Daily intraperitoneal injection
- Duration
- Variable (typically 1-4 weeks in published studies)
Mouse model; IP route; used in original discovery study to prevent diet-induced obesity
Preclinical aging study (Reynolds et al. 2021)
Clinical- Dose
- 5 mg/kg body weight
- Frequency
- Intraperitoneal injection (3 times per week or daily depending on protocol)
- Duration
- Variable (weeks to months in published studies)
Aged mouse model; demonstrated improved physical performance and metabolic parameters
MOTS-c reconstitution & storage
Reconstitute with bacteriostatic water. Add water slowly along vial wall. Do not shake. Use 1-2 mL per vial.
Lyophilized powder: Store at -20°C to 4°C. Reconstituted: Store at 2-8°C, use within 3-4 weeks. Protect from light.
MOTS-c dosing FAQ
How much bacteriostatic water should I add to a 10 mg vial of MOTS-c?
The community standard is 2 mL of bacteriostatic water for a 10 mg MOTS-c vial. That gives a concentration of 5.00 mg/mL, so a 5 mg dose is 100 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 10.00 mg/mL and halves the draw to 50 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 5 mg dose of MOTS-c?
On a standard U-100 insulin syringe, a 5 mg dose of MOTS-c is 100 units when the 10 mg vial is reconstituted with 2 mL of bacteriostatic water (5.00 mg/mL). If you use 1 mL of bac water instead, draw 50 units (10.00 mg/mL).
What is the standard MOTS-c dose?
Community-reported protocols for MOTS-c typically use 5 mg–10 mg every other day via Subcutaneous. 5 mg is a common starting dose. Most commonly reported protocol; split weekly dose across 2-3 injections, often timed around exercise
How long does a vial of MOTS-c last at 5 mg every other day?
A 10 mg vial of MOTS-c contains roughly 2 doses at 5 mg per injection. At every other day dosing, that is approximately 4 days (about 1 weeks) of supply per vial. Reconstituted MOTS-c should generally be used within 28 days when refrigerated.
Where do you inject MOTS-c?
MOTS-c is typically administered subcutaneous. Common subcutaneous sites are the lower abdomen (at least an inch from the navel), the outer thigh, and the love handles. Rotate sites with each injection to reduce localized irritation and bruising.
Can I use sterile water instead of bacteriostatic water for MOTS-c?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like MOTS-c because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on MOTS-c
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.