Humanin: Dosing Protocols
Dosing guidelines, reconstitution, and administration information
๐TL;DR
- โข2 dosing protocols documented
- โขReconstitution instructions included
- โขStorage: Store lyophilized peptide at -20 to -80 degrees C. Reconstituted solutions should be aliquoted and stored at -20 degrees C. Avoid repeated freeze-thaw cycles.
Protocol Quick-Reference
Neuroprotection and anti-aging research
Dosing
Amount
5 mg/kg/day IP (S14G-HNG analog, preclinical)
Frequency
Daily (animal studies)
Duration
3 months (primary study)
Administration
Route
SCTiming
Animal studies used intraperitoneal or intracerebroventricular routes. S14G-humanin (HNG) analog is approximately 1000-fold more potent than native humanin.
Cycle
Duration
3 months (preclinical)
Repeatable
Single cycle
โ๏ธ Suggested Bloodwork (3 tests)
CBC with differential
When: Baseline
Why: Baseline blood cell counts
CMP (Comprehensive Metabolic Panel)
When: Baseline
Why: Liver and kidney function baseline
Fasting glucose and insulin
When: Baseline
Why: Humanin affects insulin sensitivity and glucose homeostasis
๐ก Key Considerations
- โPreclinical only: no human clinical trials have been completed
- โPotential pro-tumorigenic effects via anti-apoptotic mechanism may promote cancer cell survival
- โNative humanin is rapidly degraded in vivo; S14G-HNG analog preferred for research
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| Purpose | Dose | Frequency | Duration | Notes |
|---|---|---|---|---|
| S14G-HNG Alzheimer's Model (Zhang et al., 2012) | 5 mg/kg/day intraperitoneal | Daily | 3 months | Improved spatial memory and reduced amyloid pathology in APPswe/PS1dE9 mice. S14G-humanin analog used, which is 1000-fold more potent than native humanin. |
| Preclinical Neuroprotection Studies | 0.1-10 mcg intracerebroventricular | Single or repeated dosing | Varies by study | Direct CNS delivery used in multiple preclinical models. Native humanin and HNG analog tested at various doses. |
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๐Reconstitution Instructions
Reconstitute lyophilized humanin or HNG in sterile water or PBS. Typical research concentrations range from 0.1-1 mg/mL. Gently swirl to dissolve; do not vortex. Aliquot and store frozen.
Recommended Injection Sites
- โIntraperitoneal (animal research)
- โIntracerebroventricular (animal research)
- โSubcutaneous (animal research)
๐งStorage Requirements
Store lyophilized peptide at -20 to -80 degrees C. Reconstituted solutions should be aliquoted and stored at -20 degrees C. Avoid repeated freeze-thaw cycles.
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Before You Begin
Review safety warnings and contraindications before starting any protocol.
Important Disclaimer#
Humanin is not approved for human use by any regulatory agency. There are no established human dosing protocols. All dosing information is derived from preclinical animal studies and is provided for research reference only.
Research-Based Dosing Information#
S14G-Humanin (HNG) in Alzheimer's Models#
The most extensively studied dosing protocol uses the S14G-humanin (HNG) analog, which has approximately 1000-fold greater potency than native humanin. Zhang et al. (2012) administered HNG at 5 mg/kg/day intraperitoneally to APPswe/PS1dE9 transgenic mice for 3 months, demonstrating significant improvements in spatial learning and memory along with reduced amyloid plaque burden.
Intracerebroventricular Dosing#
Several studies have administered humanin or HNG directly into the brain ventricles at doses ranging from 0.1 to 10 micrograms, demonstrating neuroprotection in models of ischemia, excitotoxicity, and amyloid-beta toxicity.
Dose Considerations#
Native humanin is rapidly degraded in vivo, requiring either higher doses or use of more stable analogs. S14G-humanin (HNG) is the preferred form for research due to its enhanced potency and somewhat improved stability.
Reconstitution Instructions#
Lyophilized humanin or HNG should be reconstituted in sterile water or phosphate-buffered saline (PBS). Gently swirl to dissolve. Typical research concentrations are 0.1-1 mg/mL. Aliquot immediately after reconstitution and store frozen.
Storage Guidelines#
Store lyophilized peptide at -20 to -80 degrees C, protected from light and moisture. Reconstituted peptide solutions should be aliquoted to avoid repeated freeze-thaw cycles and stored at -20 degrees C. Use reconstituted solutions within one month.
Dosing Context#
Humanin belongs to the Anti-Aging category of research peptides. Dosing protocols for Humanin are derived from available clinical trial data. These protocols are provided for research reference only and do not constitute medical advice. Actual dosing decisions should be made by qualified healthcare providers based on individual patient factors.
Research Protocols#
The following dosing protocols have been documented in clinical research for Humanin:
S14G-HNG Alzheimer's Model (Zhang et al., 2012)#
Dose: 5 mg/kg/day intraperitoneal
Frequency: Daily
Duration: 3 months
Improved spatial memory and reduced amyloid pathology in APPswe/PS1dE9 mice. S14G-humanin analog used, which is 1000-fold more potent than native humanin.
Preclinical Neuroprotection Studies#
Dose: 0.1-10 mcg intracerebroventricular
Frequency: Single or repeated dosing
Duration: Varies by study
Direct CNS delivery used in multiple preclinical models. Native humanin and HNG analog tested at various doses.
Reconstitution and Preparation#
Reconstitute lyophilized humanin or HNG in sterile water or PBS. Typical research concentrations range from 0.1-1 mg/mL. Gently swirl to dissolve; do not vortex. Aliquot and store frozen.
Injection Sites#
Recommended injection sites for Humanin include:
- Intraperitoneal (animal research)
- Intracerebroventricular (animal research)
- Subcutaneous (animal research)
Site rotation is recommended to minimize local tissue reactions and ensure consistent absorption.
Storage Requirements#
Store lyophilized peptide at -20 to -80 degrees C. Reconstituted solutions should be aliquoted and stored at -20 degrees C. Avoid repeated freeze-thaw cycles.
Related Reading#
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Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.