DSIP Dosing Calculator
DSIP: Delta sleep-inducing nonapeptide guide.
Pre-filled with the most-reported community reconstitution protocol for DSIP. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 5 mg vial of DSIP reconstituted with 1 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 100 mcg dose of DSIP equals 4 units on a U-100 insulin syringe (0.040 mL). DSIP is typically administered once daily via subcutaneous.
DSIP reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 100 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.020 mL | 2 units |
| 2 mL | 2.50 mg/mL | 0.040 mL | 4 units |
Calculate your DSIP dose
Alternative DSIP protocols
Beyond the default above, DSIP is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Higher-Dose Sleep Protocol
Community- Dose
- 250-500 mcg
- Frequency
- Once daily before bed
- Duration
- 2-4 weeks
- Route
- Subcutaneous
Higher dose for severe sleep issues; some users report next-day grogginess at doses above 300 mcg
Intermittent Dosing Protocol
Community- Dose
- 100-250 mcg
- Frequency
- 3 times per week
- Duration
- 4-6 weeks
- Route
- Subcutaneous
Used to minimize tolerance risk; dosed on non-consecutive evenings (e.g., Mon/Wed/Fri)
Low-Dose Stress Protocol
Community- Dose
- 50-100 mcg
- Frequency
- Once daily
- Duration
- 2-4 weeks
- Route
- Subcutaneous
Lower doses used by those targeting stress modulation rather than sleep induction
Sleep research (human clinical studies)
Clinical- Dose
- 25 nmol/kg body weight
- Frequency
- Single injection before sleep
- Duration
- Variable; studies used 1-10 administrations
Intravenous administration used in most clinical studies
Repeated administration protocol
Clinical- Dose
- 25 nmol/kg body weight
- Frequency
- Once daily before sleep
- Duration
- 10 consecutive days
Open-label studies showed sustained benefit after treatment cessation
DSIP reconstitution & storage
Reconstitute lyophilized DSIP in sterile bacteriostatic water or physiological saline. Prepare fresh solutions for each use due to limited stability in solution. Handle with standard peptide precautions.
Store lyophilized powder at -20C protected from light and moisture. Reconstituted solutions should be used promptly or stored at 2-8C for no more than 24 hours. DSIP is susceptible to degradation in solution.
DSIP dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of DSIP?
The community standard is 1 mL of bacteriostatic water for a 5 mg DSIP vial. That gives a concentration of 2.50 mg/mL, so a 100 mcg dose is 4 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 2 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 100 mcg dose of DSIP?
On a standard U-100 insulin syringe, a 100 mcg dose of DSIP is 4 units when the 5 mg vial is reconstituted with 1 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 2 units (5.00 mg/mL).
What is the standard DSIP dose?
Community-reported protocols for DSIP typically use 100 mcg–200 mcg once daily via Subcutaneous. 100 mcg is a common starting dose. Most commonly reported starting dose; injected subcutaneously in the evening before bedtime
How long does a vial of DSIP last at 100 mcg once daily?
A 5 mg vial of DSIP contains roughly 50 doses at 100 mcg per injection. At once daily dosing, that is approximately 50 days (about 7 weeks) of supply per vial. Reconstituted DSIP should generally be used within 28 days when refrigerated.
Where do you inject DSIP?
DSIP is typically injected subcutaneous. Common sites reported in the literature and by users include: Intravenous (used in most human studies), Subcutaneous (alternative route), Intranasal (explored in some research). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for DSIP?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like DSIP because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on DSIP
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.