SS-31 Dosing Calculator
SS-31 (Elamipretide): Cardiolipin-targeting mitochondrial peptide.
Pre-filled with the most-reported community reconstitution protocol for SS-31. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 10 mg vial of SS-31 reconstituted with 2 mL of bacteriostatic water yields a concentration of 5.00 mg/mL. At that concentration, a 1 mg dose of SS-31 equals 20 units on a U-100 insulin syringe (0.200 mL). SS-31 is typically administered once daily via subcutaneous.
SS-31 reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 10 mg vial.
| BAC water added | Concentration | Volume per 1 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 10.00 mg/mL | 0.100 mL | 10 units |
| 2 mL | 5.00 mg/mL | 0.200 mL | 20 units |
Calculate your SS-31 dose
Alternative SS-31 protocols
Beyond the default above, SS-31 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Low-Dose Maintenance Protocol
Community- Dose
- 0.5-1 mg
- Frequency
- 3-5 times per week
- Duration
- 8-12 weeks with breaks
- Route
- Subcutaneous
Lower frequency protocol for long-term mitochondrial support; less aggressive than daily dosing
Exercise Performance Protocol
Community- Dose
- 2-5 mg
- Frequency
- Once daily or pre-exercise
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Timed around exercise for potential mitochondrial performance enhancement; inspired by mouse exercise studies
Barth Syndrome (TAZPOWER Phase 3)
Clinical- Dose
- 40 mg subcutaneous injection
- Frequency
- Once daily
- Duration
- 12-week treatment periods (crossover design)
Pivotal Phase 3 trial dose; patients in open-label extension continued at same dose for extended periods
STEMI Cardioprotection (EMBRACE Phase 2a)
Clinical- Dose
- 0.25 mg/kg intravenous infusion
- Frequency
- Single dose at time of reperfusion
- Duration
- Single administration during PCI procedure
Administered as IV infusion beginning prior to percutaneous coronary intervention for acute STEMI
Heart Failure with Reduced Ejection Fraction (Phase 2)
Clinical- Dose
- 40 mg subcutaneous injection
- Frequency
- Once daily
- Duration
- 4 weeks
Phase 2 trial evaluating cardiac function endpoints including left ventricular volumes
SS-31 reconstitution & storage
SS-31 (elamipretide) has been supplied in clinical trials as a ready-to-use sterile solution for subcutaneous injection or as a concentrated solution for IV dilution. Clinical trial formulations were prepared by the sponsor (Stealth BioTherapeutics/Larimar Therapeutics). Specific reconstitution instructions for research-grade material are not standardized.
Clinical trial supplies were stored under refrigerated conditions (2-8 degrees Celsius). Specific storage stability data for elamipretide formulations have not been publicly disclosed. Standard peptide storage precautions apply for research-grade material: store lyophilized powder at -20 degrees Celsius and reconstituted solutions at 2-8 degrees Celsius.
SS-31 dosing FAQ
How much bacteriostatic water should I add to a 10 mg vial of SS-31?
The community standard is 2 mL of bacteriostatic water for a 10 mg SS-31 vial. That gives a concentration of 5.00 mg/mL, so a 1 mg dose is 20 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 10.00 mg/mL and halves the draw to 10 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 1 mg dose of SS-31?
On a standard U-100 insulin syringe, a 1 mg dose of SS-31 is 20 units when the 10 mg vial is reconstituted with 2 mL of bacteriostatic water (5.00 mg/mL). If you use 1 mL of bac water instead, draw 10 units (10.00 mg/mL).
What is the standard SS-31 dose?
Community-reported protocols for SS-31 typically use 1 mgâ5 mg once daily via Subcutaneous. 1 mg is a common starting dose. Most commonly discussed community protocol; dose range adapted from clinical trial data with allometric scaling
How long does a vial of SS-31 last at 1 mg once daily?
A 10 mg vial of SS-31 contains roughly 10 doses at 1 mg per injection. At once daily dosing, that is approximately 10 days (about 1 weeks) of supply per vial. Reconstituted SS-31 should generally be used within 28 days when refrigerated.
Where do you inject SS-31?
SS-31 is typically injected subcutaneous. Common sites reported in the literature and by users include: Subcutaneous (abdomen), Subcutaneous (thigh), Subcutaneous (upper arm), Intravenous (for acute care protocols). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for SS-31?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like SS-31 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
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Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.