NAD+ Dosing Calculator
NAD+ (Nicotinamide Adenine Dinucleotide): Essential coenzyme for cellular energy, DNA repair, and anti-aging research.
Pre-filled with the most-reported community reconstitution protocol for NAD+. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Also available in these vial strengths
Same NAD+ protocol, recalculated for each vial size â reconstitution volumes, concentrations, and syringe-draw units update automatically.
Quick answer · Community-reported standard
A 500 mg vial of NAD+ reconstituted with 2 mL of bacteriostatic water yields a concentration of 250.00 mg/mL. At that concentration, a 50 mg dose of NAD+ equals 20 units on a U-100 insulin syringe (0.200 mL). NAD+ is typically administered every other day via subcutaneous.
NAD+ 500 mg reconstitution and dosage: 1 mL vs 2 mL bac water
For a 500 mg vial of NAD+, bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 500 mg vial.
| BAC water added | Concentration | Volume per 50 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 500.00 mg/mL | 0.100 mL | 10 units |
| 2 mL | 250.00 mg/mL | 0.200 mL | 20 units |
NAD+ dosage chart: per-injection to weekly total
Common NAD+ protocol doses centered on the 50 mg baseline (research range 50 mgâ100 mg), with weekly totals at every other day frequency.
| Dose level | Per injection | Frequency | Weekly total |
|---|---|---|---|
| Lower | 25 mg | Every other day | 87.5 mg |
| Baseline | 50 mg | Every other day | 175 mg |
| Higher | 75 mg | Every other day | 262.5 mg |
| Upper range | 100 mg | Every other day | 350 mg |
Weekly totals assume consistent every other day dosing. Adjust the calculator below for your specific vial size and dose.
Calculate your NAD+ dose
Alternative NAD+ protocols
Beyond the default above, NAD+ is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
IV Loading Protocol
Community- Dose
- 250-500 mg
- Frequency
- 4-5 sessions over 2 weeks
- Duration
- 2 weeks (loading), then monthly maintenance
- Route
- Intravenous
Initial loading phase at IV clinics; infused slowly over 2-4 hours to minimize side effects
High-Dose IV Protocol
Community- Dose
- 500-1000 mg
- Frequency
- Weekly for 4 weeks then monthly
- Duration
- Ongoing
- Route
- Intravenous
Higher dose protocol; requires slow infusion rate (3-4 hours) to manage NAD flush symptoms
SubQ Titration Protocol
Community- Dose
- 25-100 mg (gradual increase)
- Frequency
- Twice weekly
- Duration
- 8+ weeks
- Route
- Subcutaneous
Weeks 1-2 at 25 mg, weeks 3-4 at 50 mg, weeks 5+ at 100 mg; gradual increase to manage side effects
Anti-aging and general wellness (IV NAD+)
Clinical- Dose
- 250-500 mg per session
- Frequency
- 1-2 times per week during loading; monthly for maintenance
- Duration
- Loading phase 4-8 sessions; maintenance ongoing
Infused over 2-4 hours; rate titrated to patient tolerance; nausea and flushing are rate-dependent
Neurodegenerative disease research (IV NAD+)
Clinical- Dose
- 500-1000 mg per session
- Frequency
- Daily for 7-10 days during intensive protocols
- Duration
- 7-10 day intensive courses; repeat as indicated
Higher doses used in addiction and neurology protocols; longer infusion times (4-8 hours) reduce side effects
NAD+ reconstitution & storage
IV NAD+ should be diluted in 250-500 mL normal saline and administered via slow IV infusion over 2-8 hours depending on dose and patient tolerance
IV NAD+ solutions should be prepared fresh and used within 24 hours; lyophilized NAD+ powder stored at -20C protected from light and moisture; oral NR/NMN supplements stored at room temperature in airtight containers away from heat and light
NAD+ dosing FAQ
How much bacteriostatic water should I add to a 500 mg vial of NAD+?
The community standard is 2 mL of bacteriostatic water for a 500 mg NAD+ vial. That gives a concentration of 250.00 mg/mL, so a 50 mg dose is 20 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 500.00 mg/mL and halves the draw to 10 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 50 mg dose of NAD+?
On a standard U-100 insulin syringe, a 50 mg dose of NAD+ is 20 units when the 500 mg vial is reconstituted with 2 mL of bacteriostatic water (250.00 mg/mL). If you use 1 mL of bac water instead, draw 10 units (500.00 mg/mL).
What is the standard NAD+ dose?
Community-reported protocols for NAD+ typically use 50 mgâ100 mg every other day via Subcutaneous. 50 mg is a common starting dose. Home-use alternative to IV; gradual titration from 25 mg recommended to assess tolerance
How long does a vial of NAD+ last at 50 mg every other day?
A 500 mg vial of NAD+ contains roughly 10 doses at 50 mg per injection. At every other day dosing, that is approximately 20 days (about 3 weeks) of supply per vial. Reconstituted NAD+ should generally be used within 28 days when refrigerated.
Where do you inject NAD+?
NAD+ is typically injected subcutaneous. Common sites reported in the literature and by users include: Antecubital vein (IV infusion), Any peripheral IV access site. Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for NAD+?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like NAD+ because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on NAD+
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.