MGF Dosing Calculator
MGF: Mechano Growth Factor for muscle repair.
Pre-filled with the most-reported community reconstitution protocol for MGF. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 5 mg vial of MGF reconstituted with 2 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 200 mcg dose of MGF equals 8 units on a U-100 insulin syringe (0.080 mL). MGF is typically administered once daily via intramuscular (site-specific).
MGF reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 200 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.040 mL | 4 units |
| 2 mL | 2.50 mg/mL | 0.080 mL | 8 units |
Calculate your MGF dose
Alternative MGF protocols
Beyond the default above, MGF is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
MGF/IGF-1 LR3 Alternating Protocol
Community- Dose
- 200-400 mcg MGF on MGF days
- Frequency
- Alternating days with IGF-1 LR3
- Duration
- 10-20 day cycles
- Route
- Intramuscular
MGF on training days for satellite cell activation, IGF-1 LR3 on rest days for growth signaling
Conservative SubQ Protocol
Community- Dose
- 100-200 mcg
- Frequency
- Post-workout
- Duration
- 4-6 weeks
- Route
- Subcutaneous
SubQ injection for those uncomfortable with IM; effectiveness debated due to very short half-life
Preclinical muscle repair (mouse model)
Clinical- Dose
- 0.5-5 mcg per injection site (intramuscular)
- Frequency
- Single injection or daily for 3-5 days
- Duration
- Acute administration (1-5 days post-injury)
Most preclinical muscle studies use local intramuscular injection at the site of damage. Doses range from 0.5 to 5 mcg depending on the model. The extremely short half-life (~5-7 minutes) limits systemic exposure.
Preclinical cardiac repair (mouse MI model)
Clinical- Dose
- 100-300 ng intravenous or intracoronary
- Frequency
- Single dose at time of injury
- Duration
- Single administration
Carpenter et al. (2008) used systemic IV delivery of MGF E-domain peptide following acute MI. Some studies used localized delivery via polymeric microstructures for sustained local release.
Preclinical bone healing (rabbit model)
Clinical- Dose
- 10-50 mcg locally applied
- Frequency
- Single application at bone defect site
- Duration
- Single administration
Liu et al. (2010) applied MGF E-domain peptide directly to bone defect sites in rabbits. Local application allows direct peptide contact with osteoblast progenitor cells.
MGF reconstitution & storage
Reconstitute lyophilized MGF with sterile bacteriostatic water or sterile water for injection. For a typical 2 mg vial, add 1-2 mL of diluent for a concentration of 1-2 mg/mL. Gently swirl (do not shake) until fully dissolved. Solution should be clear and colorless. Use immediately after reconstitution due to the peptide's instability. Aliquot into single-use portions if not using entire vial immediately.
Store lyophilized powder at -20C for long-term storage. Protect from light and moisture. Reconstituted solution should be stored at 2-8C and used within 24-48 hours due to the peptide's inherent instability. Do not freeze reconstituted solutions. Do not use if solution appears cloudy or contains particulate matter.
MGF dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of MGF?
The community standard is 2 mL of bacteriostatic water for a 5 mg MGF vial. That gives a concentration of 2.50 mg/mL, so a 200 mcg dose is 8 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 4 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 200 mcg dose of MGF?
On a standard U-100 insulin syringe, a 200 mcg dose of MGF is 8 units when the 5 mg vial is reconstituted with 2 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 4 units (5.00 mg/mL).
What is the standard MGF dose?
Community-reported protocols for MGF typically use 200 mcg–400 mcg once daily via Intramuscular (site-specific). 200 mcg is a common starting dose. Injected bilaterally into muscles just trained (e.g. 100-200 mcg per side); very short half-life (~5-7 minutes) requires immediate post-exercise timing
How long does a vial of MGF last at 200 mcg once daily?
A 5 mg vial of MGF contains roughly 25 doses at 200 mcg per injection. At once daily dosing, that is approximately 25 days (about 4 weeks) of supply per vial. Reconstituted MGF should generally be used within 28 days when refrigerated.
Where do you inject MGF?
MGF is typically injected intramuscular (site-specific). Common sites reported in the literature and by users include: Intramuscular (at site of target tissue, for local effect), Subcutaneous (abdomen, for systemic distribution), Subcutaneous (near target tissue, for localized effect). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for MGF?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like MGF because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
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Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.