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IGF-1 LR3 Dosing Calculator

IGF-1 LR3: Long-acting IGF-1 analog with 2-3x extended half-life.

Pre-filled with the most-reported community reconstitution protocol for IGF-1 LR3. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.

Quick answer · Community-reported standard

A 5 mg vial of IGF-1 LR3 reconstituted with 1 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 20 mcg dose of IGF-1 LR3 equals 0.8 units on a U-100 insulin syringe (0.008 mL). IGF-1 LR3 is typically administered once daily via subcutaneous or intramuscular.

IGF-1 LR3 reconstitution: 1 mL vs 2 mL bac water

Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.

BAC water addedConcentrationVolume per 20 mcg doseDraw on U-100 syringe (units)
1 mL5.00 mg/mL0.004 mL0.4 units
2 mL2.50 mg/mL0.008 mL0.8 units
Based on a 5 mg vial of IGF-1 LR3 reconstituted with bacteriostatic water. Insulin-syringe units assume a standard U-100 syringe (100 units = 1 mL).

Calculate your IGF-1 LR3 dose

Alternative IGF-1 LR3 protocols

Beyond the default above, IGF-1 LR3 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.

Conservative/Beginner Protocol

Community
Dose
20-40 mcg
Frequency
Once daily
Duration
4 weeks
Route
Subcutaneous

Lower-dose SubQ protocol for first-time users; start at 20 mcg and titrate up based on tolerance

Advanced Bodybuilding Protocol

Community
Dose
50-100 mcg
Frequency
Once daily post-workout
Duration
4-6 weeks
Route
Intramuscular (site-specific)

Higher-dose protocol split across 2-4 muscle groups post-workout. Carries higher hypoglycemia risk.

10-Day Alternating MGF/IGF-1 LR3 Protocol

Community
Dose
40-50 mcg IGF-1 LR3 on alternating days
Frequency
Every other day (alternating with MGF)
Duration
10-20 days per cycle
Route
Intramuscular

Alternates IGF-1 LR3 and MGF to target different phases of muscle repair and satellite cell activation

Cell culture supplementation (serum-free media)

Clinical
Dose
50-100 ng/mL
Frequency
Added at media change; typically every 2-3 days depending on cell type and culture conditions
Duration
Duration of cell culture experiment

Standard concentration range for mammalian cell culture. Lower concentrations (10-20 ng/mL) may be sufficient for some cell types. Higher concentrations (up to 200 ng/mL) used in some specialized applications.

Cell culture supplementation (serum-containing media)

Clinical
Dose
50-100 ng/mL
Frequency
Added at media change
Duration
Duration of cell culture experiment

In serum-containing media, IGFBPs present in serum will sequester some native IGF-1 but have minimal effect on IGF-1 LR3 activity, which is the primary advantage of using this analog over native IGF-1.

IGF-1 LR3 reconstitution & storage

Reconstitute lyophilized IGF-1 LR3 by adding sterile 10 mM HCl or sterile water to the vial. Allow the solution to sit for several minutes, then gently swirl to dissolve. Do not vortex vigorously. A typical stock concentration is 1 mg/mL (1000 mcg/mL). Aliquot into single-use volumes to avoid repeated freeze-thaw cycles. For cell culture use, further dilute stock solution into culture media to achieve the desired working concentration (typically 50-100 ng/mL). Use sterile filtered (0.22 micron) solutions.

Lyophilized powder: Store at -20 to -80 degrees C, protected from moisture. Reconstituted stock solution: Aliquot and store at -20 degrees C; avoid repeated freeze-thaw cycles. Working dilutions in culture media should be prepared fresh. Reconstituted solutions stored at 2-8 degrees C should be used within 1 week.

IGF-1 LR3 dosing FAQ

How much bacteriostatic water should I add to a 5 mg vial of IGF-1 LR3?

The community standard is 1 mL of bacteriostatic water for a 5 mg IGF-1 LR3 vial. That gives a concentration of 2.50 mg/mL, so a 20 mcg dose is 0.8 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 0.4 units. Less water = smaller injection volume but harder to measure precisely.

How many syringe units is a 20 mcg dose of IGF-1 LR3?

On a standard U-100 insulin syringe, a 20 mcg dose of IGF-1 LR3 is 0.8 units when the 5 mg vial is reconstituted with 1 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 0.4 units (5.00 mg/mL).

What is the standard IGF-1 LR3 dose?

Community-reported protocols for IGF-1 LR3 typically use 20 mcg–50 mcg once daily via Subcutaneous or Intramuscular. 20 mcg is a common starting dose. Injected post-workout bilaterally into trained muscle groups or subcutaneously. Strict 4-6 week cycles to prevent desensitization.

How long does a vial of IGF-1 LR3 last at 20 mcg once daily?

A 5 mg vial of IGF-1 LR3 contains roughly 250 doses at 20 mcg per injection. At once daily dosing, that is approximately 250 days (about 36 weeks) of supply per vial. Reconstituted IGF-1 LR3 should generally be used within 28 days when refrigerated.

Where do you inject IGF-1 LR3?

IGF-1 LR3 is typically administered subcutaneous or intramuscular. Common subcutaneous sites are the lower abdomen (at least an inch from the navel), the outer thigh, and the love handles. Rotate sites with each injection to reduce localized irritation and bruising.

Can I use sterile water instead of bacteriostatic water for IGF-1 LR3?

Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like IGF-1 LR3 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.

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Medical Disclaimer

This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.