FOXO4-DRI Dosing Calculator

Dose

2 mg

Frequency

Every other day for 5 doses (10 days total)

Duration

Single course repeated annually

Route

Subcutaneous

Longer course used by some biohackers; total dose of 10 mg per course

Dose

1 mg

Frequency

Every other day for 3 doses

Duration

Single course

Route

Subcutaneous

Conservative approach for first-time users; lower cost entry point for an expensive peptide

Dose

5 mg/kg intravenous

Frequency

Three times per week

Duration

Several weeks (exact total duration varied by experiment)

Administered to mice over 24 months old; demonstrated improved fur density, exploratory behavior, and renal function

Dose

5 mg/kg intravenous

Frequency

Three times per week

Duration

Treatment period following doxorubicin-induced senescence

Demonstrated improved recovery and reduced tissue damage in mice with chemotherapy-induced senescent cell burden

How much bacteriostatic water should I add to a 5 mg vial of FOXO4-DRI?

The community standard is 2 mL of bacteriostatic water for a 5 mg FOXO4-DRI vial. That gives a concentration of 2.50 mg/mL, so a 2 mg dose is 80 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 40 units. Less water = smaller injection volume but harder to measure precisely.

How many syringe units is a 2 mg dose of FOXO4-DRI?

On a standard U-100 insulin syringe, a 2 mg dose of FOXO4-DRI is 80 units when the 5 mg vial is reconstituted with 2 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 40 units (5.00 mg/mL).

What is the standard FOXO4-DRI dose?

Community-reported protocols for FOXO4-DRI typically use 2 mg every other day via Subcutaneous. 2 mg is a common starting dose. Most commonly cited community protocol; derived from Baar et al. mouse study scaling; expensive peptide

How long does a vial of FOXO4-DRI last at 2 mg every other day?

A 5 mg vial of FOXO4-DRI contains roughly 2 doses at 2 mg per injection. At every other day dosing, that is approximately 4 days (about 1 weeks) of supply per vial. Reconstituted FOXO4-DRI should generally be used within 28 days when refrigerated.

Where do you inject FOXO4-DRI?

FOXO4-DRI is typically administered subcutaneous. Common subcutaneous sites are the lower abdomen (at least an inch from the navel), the outer thigh, and the love handles. Rotate sites with each injection to reduce localized irritation and bruising.

Can I use sterile water instead of bacteriostatic water for FOXO4-DRI?

Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like FOXO4-DRI because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.