Rusfertide Dosing Calculator
Rusfertide (PTG-300): hepcidin mimetic peptide for polycythemia vera.
Pre-filled with the most-reported community reconstitution protocol for Rusfertide. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 15 mg vial of Rusfertide reconstituted with 2 mL of bacteriostatic water yields a concentration of 7.50 mg/mL. At that concentration, a 40 mg dose of Rusfertide equals 533.3 units on a U-100 insulin syringe (5.333 mL). Rusfertide is typically administered once weekly via subcutaneous injection.
Rusfertide reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 15 mg vial.
| BAC water added | Concentration | Volume per 40 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 15.00 mg/mL | 2.667 mL | 266.7 units |
| 2 mL | 7.50 mg/mL | 5.333 mL | 533.3 units |
Calculate your Rusfertide dose
Alternative Rusfertide protocols
Beyond the default above, Rusfertide is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Polycythemia Vera (Erythrocytosis Control)
Clinical- Dose
- 20 mg subcutaneously once weekly as starting dose. Titrate in the range of 10-120 mg weekly to maintain hematocrit <45%.
- Frequency
- Once weekly subcutaneous injection
- Duration
- Ongoing (treatment interruption leads to loss of hematocrit control)
Mean final weekly dose in REVIVE was 41.3 mg. Female patients and those with lower body weight may require lower doses. Dose adjustments guided by hematocrit monitoring.
Rusfertide reconstitution & storage
Rusfertide has been studied in both lyophilized powder (requiring reconstitution) and aqueous formulations for subcutaneous injection. The specific formulation depends on the clinical trial or eventual commercial product.
Storage conditions depend on formulation. Lyophilized powder: store at 2-8 degrees C. Aqueous formulation: store refrigerated. Consult specific product labeling.
Rusfertide dosing FAQ
How much bacteriostatic water should I add to a 15 mg vial of Rusfertide?
The community standard is 2 mL of bacteriostatic water for a 15 mg Rusfertide vial. That gives a concentration of 7.50 mg/mL, so a 40 mg dose is 533.3 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 15.00 mg/mL and halves the draw to 266.7 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 40 mg dose of Rusfertide?
On a standard U-100 insulin syringe, a 40 mg dose of Rusfertide is 533.3 units when the 15 mg vial is reconstituted with 2 mL of bacteriostatic water (7.50 mg/mL). If you use 1 mL of bac water instead, draw 266.7 units (15.00 mg/mL).
What is the standard Rusfertide dose?
Community-reported protocols for Rusfertide typically use 40 mgâ80 mg once weekly via Subcutaneous injection. 40 mg is a common starting dose. Phase 3 clinical trial protocol; not yet commercially available; FDA Breakthrough Therapy Designation
Where do you inject Rusfertide?
Rusfertide is typically injected subcutaneous injection. Common sites reported in the literature and by users include: Abdomen, Thigh. Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for Rusfertide?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Rusfertide because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on Rusfertide
Rusfertide overview
Mechanism of action, primary uses, and research summary
Rusfertide dosing protocols
Full clinical and research-based dosing reference
Rusfertide community protocols
Real-world usage patterns from peptide communities
Rusfertide side effects
Reported adverse effects and safety considerations
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.