Glutathione Dosing Calculator
Pre-filled with the most-reported community reconstitution protocol for Glutathione. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 200 mg vial of Glutathione reconstituted with 2 mL of bacteriostatic water yields a concentration of 100.00 mg/mL. At that concentration, a 600 mg dose of Glutathione equals 600 units on a U-100 insulin syringe (6.000 mL). Glutathione is typically administered twice weekly via intravenous.
Glutathione reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 200 mg vial.
| BAC water added | Concentration | Volume per 600 mg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 200.00 mg/mL | 3.000 mL | 300 units |
| 2 mL | 100.00 mg/mL | 6.000 mL | 600 units |
Calculate your Glutathione dose
Alternative Glutathione protocols
Beyond the default above, Glutathione is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Subcutaneous Injection Protocol
Community- Dose
- 200-400 mg
- Frequency
- 2-3 times weekly
- Duration
- 4-8 weeks
- Route
- Subcutaneous
Home-use alternative to IV; uses compounded reduced glutathione
Intramuscular Injection Protocol
Community- Dose
- 200 mg
- Frequency
- 1-2 times weekly
- Duration
- Ongoing
- Route
- Intramuscular
Standard IM glutathione injection; often combined with B-vitamin injections
Nebulized Glutathione Protocol
Community- Dose
- 200-400 mg
- Frequency
- 1-2 times daily
- Duration
- 2-4 weeks
- Route
- Inhalation
Used for respiratory and detoxification purposes; controversial due to potential airway irritation
Antioxidant supplementation (oral)
Clinical- Dose
- 250-500 mg daily in divided doses
- Frequency
- Once or twice daily
- Duration
- 4-12 weeks
Standard oral GSH has low bioavailability; liposomal formulations may improve absorption
Skin lightening (oral/topical combination)
Clinical- Dose
- 500-1200 mg daily oral; 2% topical serum
- Frequency
- Oral twice daily; topical once daily
- Duration
- 8-12 weeks
Combination topical plus oral showed best results in RCTs; effects are modest and may reverse after discontinuation
Glutathione reconstitution & storage
Lyophilized glutathione for injection should be reconstituted with sterile water or bacteriostatic water per manufacturer instructions
Store lyophilized powder at 2-8°C protected from light; reconstituted solution should be used promptly; oral supplements stored at room temperature in airtight containers; include chelators (EDTA) to prevent metal-catalyzed oxidation
Glutathione dosing FAQ
How much bacteriostatic water should I add to a 200 mg vial of Glutathione?
The community standard is 2 mL of bacteriostatic water for a 200 mg Glutathione vial. That gives a concentration of 100.00 mg/mL, so a 600 mg dose is 600 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 200.00 mg/mL and halves the draw to 300 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 600 mg dose of Glutathione?
On a standard U-100 insulin syringe, a 600 mg dose of Glutathione is 600 units when the 200 mg vial is reconstituted with 2 mL of bacteriostatic water (100.00 mg/mL). If you use 1 mL of bac water instead, draw 300 units (200.00 mg/mL).
What is the standard Glutathione dose?
Community-reported protocols for Glutathione typically use 600 mg–1200 mg twice weekly via Intravenous. 600 mg is a common starting dose. Most common clinical/community protocol; administered as IV push over 10-15 minutes at wellness clinics
Where do you inject Glutathione?
Glutathione is typically injected intravenous. Common sites reported in the literature and by users include: Deltoid (IM), Abdomen (SC), Gluteal (IM). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for Glutathione?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Glutathione because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on Glutathione
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.