ACE-031 Dosing Calculator
Pre-filled with the most-reported community reconstitution protocol for ACE-031. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Also available in these vial strengths
Same ACE-031 protocol, recalculated for each vial size — reconstitution volumes, concentrations, and syringe-draw units update automatically.
Quick answer · Community-reported standard
A 15 mg vial of ACE-031 reconstituted with 2 mL of bacteriostatic water yields a concentration of 7.50 mg/mL. At that concentration, a 500 mcg dose of ACE-031 equals 6.7 units on a U-100 insulin syringe (0.067 mL). ACE-031 is typically administered twice weekly via subcutaneous injection.
ACE-031 15 mg reconstitution and dosage: 1 mL vs 2 mL bac water
For a 15 mg vial of ACE-031, bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 15 mg vial.
| BAC water added | Concentration | Volume per 500 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 15.00 mg/mL | 0.033 mL | 3.3 units |
| 2 mL | 7.50 mg/mL | 0.067 mL | 6.7 units |
ACE-031 dosage chart: per-injection to weekly total
Common ACE-031 protocol doses centered on the 500 mcg baseline (research range 0.5 mg–3 mg), with weekly totals at twice weekly frequency.
| Dose level | Per injection | Frequency | Weekly total |
|---|---|---|---|
| Lower | 0.25 mg | Twice weekly | 0.5 mg |
| Baseline | 0.5 mg | Twice weekly | 1 mg |
| Higher | 0.75 mg | Twice weekly | 1.5 mg |
| Upper range | 1 mg | Twice weekly | 2 mg |
Weekly totals assume consistent twice weekly dosing. Adjust the calculator below for your specific vial size and dose.
Calculate your ACE-031 dose
Alternative ACE-031 protocols
Beyond the default above, ACE-031 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Phase 1 single ascending dose (healthy volunteers)
Clinical- Dose
- 0.02 to 3.0 mg/kg subcutaneous injection
- Frequency
- Single dose
- Duration
- Single administration with follow-up monitoring
Dose-dependent lean body mass increases observed at 1 and 3 mg/kg; highest dose (3 mg/kg) produced approximately 1 kg lean mass increase
Phase 2 DMD study (discontinued)
Clinical- Dose
- 1.0 to 2.5 mg/kg subcutaneous injection
- Frequency
- Every 2 weeks
- Duration
- Planned 12-week course; terminated early due to safety concerns
Study terminated by DSMB due to vascular adverse events (epistaxis, telangiectasias); dose escalation was not completed
ACE-031 reconstitution & storage
ACE-031 was supplied as a sterile solution for subcutaneous injection in clinical trials. As a discontinued investigational compound, no commercial formulation exists. Research-grade material requires verification of protein integrity, sterility, and endotoxin content before use.
ACE-031 protein solutions should be stored at 2-8 degrees Celsius (refrigerated). Protect from freezing and agitation. Protein solutions are sensitive to repeated freeze-thaw cycles which can cause aggregation and loss of activity.
ACE-031 dosing FAQ
How much bacteriostatic water should I add to a 15 mg vial of ACE-031?
The community standard is 2 mL of bacteriostatic water for a 15 mg ACE-031 vial. That gives a concentration of 7.50 mg/mL, so a 500 mcg dose is 6.7 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 15.00 mg/mL and halves the draw to 3.3 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 500 mcg dose of ACE-031?
On a standard U-100 insulin syringe, a 500 mcg dose of ACE-031 is 6.7 units when the 15 mg vial is reconstituted with 2 mL of bacteriostatic water (7.50 mg/mL). If you use 1 mL of bac water instead, draw 3.3 units (15.00 mg/mL).
What is the standard ACE-031 dose?
Community-reported protocols for ACE-031 typically use 0.5 mg–3 mg twice weekly via Subcutaneous injection. 500 mcg is a common starting dose. Based on published Phase 1/2 trial data; development halted in 2013 due to safety concerns
How long does a vial of ACE-031 last at 500 mcg twice weekly?
A 15 mg vial of ACE-031 contains roughly 30 doses at 500 mcg per injection. At twice weekly dosing, that is approximately 105 days (about 15 weeks) of supply per vial. Reconstituted ACE-031 should generally be used within 28 days when refrigerated.
Where do you inject ACE-031?
ACE-031 is typically injected subcutaneous injection. Common sites reported in the literature and by users include: Subcutaneous (abdomen), Subcutaneous (thigh). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for ACE-031?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like ACE-031 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on ACE-031
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.