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Vilon Dosing Calculator

Vilon (Lys-Glu): synthetic dipeptide bioregulator with immunomodulatory and geroprotective effects.

Pre-filled with the most-reported community reconstitution protocol for Vilon. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.

Quick answer · Community-reported standard

A 5 mg vial of Vilon reconstituted with 1 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 100 mcg dose of Vilon equals 4 units on a U-100 insulin syringe (0.040 mL). Vilon is typically administered once daily via subcutaneous.

Vilon reconstitution: 1 mL vs 2 mL bac water

Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.

BAC water addedConcentrationVolume per 100 mcg doseDraw on U-100 syringe (units)
1 mL5.00 mg/mL0.020 mL2 units
2 mL2.50 mg/mL0.040 mL4 units
Based on a 5 mg vial of Vilon reconstituted with bacteriostatic water. Insulin-syringe units assume a standard U-100 syringe (100 units = 1 mL).

Calculate your Vilon dose

Alternative Vilon protocols

Beyond the default above, Vilon is also reported in the following protocols. Use these as starting points and recalculate with the tool above.

Oral Bioregulator Capsule Protocol

Community
Dose
1-2 capsules daily
Frequency
1-2 times daily with meals
Duration
10-30 days, repeated every 4-6 months
Route
Oral

Most accessible form; available as capsules from bioregulator suppliers, following standard Khavinson cycling

Preclinical Geroprotective Research (Subcutaneous)

Clinical
Dose
In CBA mouse studies by Khavinson and Anisimov, vilon was administered subcutaneously starting at 6 months of age. Chronic administration extended lifespan and reduced spontaneous tumor incidence.
Frequency
Chronic subcutaneous injection in preclinical models
Duration
Long-term administration in mouse lifespan studies

Published in Doklady Biological Sciences (2000) and Bulletin of Experimental Biology and Medicine (2000). Mouse doses are not translatable to human dosing. No human PK data exists.

Russian Bioregulator Supplement (Oral Capsules)

Clinical
Dose
Vilon has been marketed in Russia as an oral bioregulator supplement in capsule form. Specific dosing recommendations from Russian manufacturers are not validated by Western regulatory standards.
Frequency
Not established by Western clinical trials
Duration
Not established by Western clinical trials

No formal pharmacokinetic studies demonstrating oral bioavailability of intact vilon dipeptide have been published. Efficacy of oral capsule formulations has not been demonstrated in controlled clinical trials.

Vilon reconstitution & storage

Research-grade vilon is supplied as lyophilized powder. Reconstitute in sterile water or appropriate buffer for in vitro or in vivo research use. Follow supplier-specific instructions. Also available as pre-formulated oral capsules from Russian supplement manufacturers.

Store lyophilized peptide at -20 degrees C, protected from light and moisture. Reconstituted solutions should be used promptly or aliquoted and stored at -20 degrees C. Capsule formulations should be stored per manufacturer instructions.

Vilon dosing FAQ

How much bacteriostatic water should I add to a 5 mg vial of Vilon?

The community standard is 1 mL of bacteriostatic water for a 5 mg Vilon vial. That gives a concentration of 2.50 mg/mL, so a 100 mcg dose is 4 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 2 units. Less water = smaller injection volume but harder to measure precisely.

How many syringe units is a 100 mcg dose of Vilon?

On a standard U-100 insulin syringe, a 100 mcg dose of Vilon is 4 units when the 5 mg vial is reconstituted with 1 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 2 units (5.00 mg/mL).

What is the standard Vilon dose?

Community-reported protocols for Vilon typically use 100 mcg–500 mcg once daily via Subcutaneous. 100 mcg is a common starting dose. Injectable form for potentially more reliable delivery; doses very low given the dipeptide simplicity

How long does a vial of Vilon last at 100 mcg once daily?

A 5 mg vial of Vilon contains roughly 50 doses at 100 mcg per injection. At once daily dosing, that is approximately 50 days (about 7 weeks) of supply per vial. Reconstituted Vilon should generally be used within 28 days when refrigerated.

Where do you inject Vilon?

Vilon is typically administered subcutaneous. Common subcutaneous sites are the lower abdomen (at least an inch from the navel), the outer thigh, and the love handles. Rotate sites with each injection to reduce localized irritation and bruising.

Can I use sterile water instead of bacteriostatic water for Vilon?

Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Vilon because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.

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Medical Disclaimer

This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.