Vesugen Dosing Calculator
Vesugen (KED, Lys-Glu-Asp, T-38) is an unapproved Khavinson tripeptide studied for vascular-endothelial and SIRT1-related effects.
Pre-filled with the most-reported clinical reconstitution protocol for Vesugen. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Clinical protocol
A 5 mg vial of Vesugen reconstituted with 2 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 400 mcg dose of Vesugen equals 16 units on a U-100 insulin syringe (0.160 mL). Vesugen is typically administered once daily.
Vesugen reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 400 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.080 mL | 8 units |
| 2 mL | 2.50 mg/mL | 0.160 mL | 16 units |
Calculate your Vesugen dose
Alternative Vesugen protocols
Beyond the default above, Vesugen is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Reported Khavinson bioregulation-clinic course
Clinical- Dose
- Low microgram per kilogram range (oral / sublingual)
- Frequency
- Daily during course
- Duration
- 10-20 days, then extended off-period
Reported from Russian clinic practice; not PubMed-indexed; not validated by randomised trial.
Community-reported subcutaneous protocol
Clinical- Dose
- 1-2 mg per injection (community-reported)
- Frequency
- Daily or every other day
- Duration
- 10-20 day courses
Community-sourced from forums and vendor packaging; no PubMed-indexed pharmacokinetic basis. Subcutaneous bioavailability of KED in humans has not been characterised.
Vesugen reconstitution & storage
Vesugen is typically sold as a lyophilised powder. Reconstitution with bacteriostatic 0.9% sodium chloride is the standard short-peptide handling. No FDA-recognised cGMP manufacturer produces Vesugen.
Lyophilised powder: refrigerate (2-8 C) for long-term storage. Reconstituted solution: refrigerate and use within 14-28 days per general short-peptide guidance. No Vesugen-specific stability data are available in PubMed-indexed literature.
Vesugen dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of Vesugen?
The community standard is 2 mL of bacteriostatic water for a 5 mg Vesugen vial. That gives a concentration of 2.50 mg/mL, so a 400 mcg dose is 16 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 8 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 400 mcg dose of Vesugen?
On a standard U-100 insulin syringe, a 400 mcg dose of Vesugen is 16 units when the 5 mg vial is reconstituted with 2 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 8 units (5.00 mg/mL).
What is the standard Vesugen dose?
Clinical protocols for Vesugen typically use 400 mcg once daily via subcutaneous injection. 400 mcg is a common starting dose. Reference dose from Khavinson 2021 (PMID 34071923). Mouse model, not validated for human translation. KED was co-administered with EDR for most endpoints.
How long does a vial of Vesugen last at 400 mcg once daily?
A 5 mg vial of Vesugen contains roughly 12 doses at 400 mcg per injection. At once daily dosing, that is approximately 12 days (about 2 weeks) of supply per vial. Reconstituted Vesugen should generally be used within 28 days when refrigerated.
Where do you inject Vesugen?
Vesugen is typically injected subcutaneously. Common sites reported in the literature and by users include: Abdominal subcutaneous tissue (community-reported), Anterior thigh subcutaneous tissue (community-reported). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for Vesugen?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like Vesugen because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on Vesugen
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.