AOD-9604 Dosing Calculator
AOD-9604: HGH fragment for fat loss research.
Pre-filled with the most-reported community reconstitution protocol for AOD-9604. See how 1 mL and 2 mL of bacteriostatic water change concentration and syringe-draw volume, then adjust the calculator below for your own vial.
Quick answer · Community-reported standard
A 5 mg vial of AOD-9604 reconstituted with 2 mL of bacteriostatic water yields a concentration of 2.50 mg/mL. At that concentration, a 300 mcg dose of AOD-9604 equals 12 units on a U-100 insulin syringe (0.120 mL). AOD-9604 is typically administered once daily via subcutaneous.
AOD-9604 reconstitution: 1 mL vs 2 mL bac water
Bacteriostatic water volume is flexible. Less water means higher concentration and smaller injection volumes; more water means lower concentration and easier dose precision. Both options below are valid for a 5 mg vial.
| BAC water added | Concentration | Volume per 300 mcg dose | Draw on U-100 syringe (units) |
|---|---|---|---|
| 1 mL | 5.00 mg/mL | 0.060 mL | 6 units |
| 2 mL | 2.50 mg/mL | 0.120 mL | 12 units |
Calculate your AOD-9604 dose
Alternative AOD-9604 protocols
Beyond the default above, AOD-9604 is also reported in the following protocols. Use these as starting points and recalculate with the tool above.
Split Dose Protocol
Community- Dose
- 250 mcg per injection
- Frequency
- Twice daily (AM fasted + pre-bed)
- Duration
- 12-16 weeks
- Route
- Subcutaneous
Total 500 mcg daily split between morning and evening; both injections on empty stomach
High-Dose Protocol
Community- Dose
- 500 mcg
- Frequency
- Once daily
- Duration
- 8-12 weeks
- Route
- Subcutaneous
Higher dose used by some clinics; based on the upper range of clinical trial data
Oral AOD-9604 Protocol
Community- Dose
- 300-600 mcg
- Frequency
- Once daily
- Duration
- 12+ weeks
- Route
- Oral
Oral formulation gaining popularity; AOD-9604 was originally studied via oral route in some trials. GRAS status in the US as a food supplement.
Obesity research (oral, from clinical trials)
Clinical- Dose
- 1 mg per day (oral)
- Frequency
- Once daily
- Duration
- 12-24 weeks (from clinical trial protocols)
Based on Phase IIa/IIb clinical trial protocols. Oral dosing was used in all human clinical trials. The Phase IIa trial used 1 mg/day orally. The Phase IIb OPTIONS study tested lower doses over 24 weeks. AOD-9604 failed to achieve statistically significant weight loss at these doses in the pivotal trial.
Fat metabolism research (subcutaneous)
Clinical- Dose
- 250-300 mcg per day
- Frequency
- Once daily, typically morning on an empty stomach
- Duration
- 4-12 weeks per research cycle
Subcutaneous dosing is commonly discussed in research settings but was not the route used in human clinical trials (which used oral dosing). No published clinical trial data for subcutaneous AOD-9604 in humans. Doses derived from anecdotal research protocols, not controlled studies.
AOD-9604 reconstitution & storage
AOD-9604 is supplied as a lyophilized powder. Reconstitute with bacteriostatic water for injection. For a 5 mg vial, add 2.5 mL bacteriostatic water to yield a concentration of 2 mg/mL (2000 mcg/mL). For a 250 mcg dose, withdraw 0.125 mL (12.5 units on a standard insulin syringe). Gently swirl the vial to dissolve; do not shake vigorously as this may damage the disulfide bond structure. Ensure complete dissolution before use.
Store lyophilized AOD-9604 at -20 degrees Celsius in a sealed, desiccated container. Once reconstituted, refrigerate at 2-8 degrees Celsius and use within 4 weeks. Do not freeze reconstituted solution. Protect from light and avoid exposure to strong reducing agents that could break the disulfide bond. The disulfide bond between Cys7 and Cys14 is essential for biological activity; degradation may not be visually apparent.
AOD-9604 dosing FAQ
How much bacteriostatic water should I add to a 5 mg vial of AOD-9604?
The community standard is 2 mL of bacteriostatic water for a 5 mg AOD-9604 vial. That gives a concentration of 2.50 mg/mL, so a 300 mcg dose is 12 units on a U-100 insulin syringe. Adding 1 mL instead doubles the concentration to 5.00 mg/mL and halves the draw to 6 units. Less water = smaller injection volume but harder to measure precisely.
How many syringe units is a 300 mcg dose of AOD-9604?
On a standard U-100 insulin syringe, a 300 mcg dose of AOD-9604 is 12 units when the 5 mg vial is reconstituted with 2 mL of bacteriostatic water (2.50 mg/mL). If you use 1 mL of bac water instead, draw 6 units (5.00 mg/mL).
What is the standard AOD-9604 dose?
Community-reported protocols for AOD-9604 typically use 300 mcg once daily via Subcutaneous. 300 mcg is a common starting dose. Most commonly reported protocol; injected on empty stomach in the morning. Based on clinical trial dose.
How long does a vial of AOD-9604 last at 300 mcg once daily?
A 5 mg vial of AOD-9604 contains roughly 16 doses at 300 mcg per injection. At once daily dosing, that is approximately 16 days (about 2 weeks) of supply per vial. Reconstituted AOD-9604 should generally be used within 28 days when refrigerated.
Where do you inject AOD-9604?
AOD-9604 is typically injected subcutaneous. Common sites reported in the literature and by users include: Abdomen (subcutaneous, most common research site), Thigh (subcutaneous, alternative site), Intra-articular (for cartilage research, requires medical supervision). Rotate sites with each injection to reduce localized irritation.
Can I use sterile water instead of bacteriostatic water for AOD-9604?
Bacteriostatic water (containing 0.9% benzyl alcohol as a preservative) is strongly preferred for multi-dose vials like AOD-9604 because it inhibits microbial growth and lets a single vial be used over multiple weeks. Sterile water has no preservative and should generally be reserved for single-use preparations only.
More on AOD-9604
Medical Disclaimer
This website is for educational and informational purposes only. The information provided is not intended to diagnose, treat, cure, or prevent any disease. Always consult with a qualified healthcare professional before using any peptide or supplement.